Juan Martin Arnoletti is currently a fourth-year combined BS/MPH student at the University of Florida with a major in Microbiology (Spring 2022) and concentrating in Epidemiology (Spring 2023). Martin joined the Brusko lab in the Fall of 2019 as an undergraduate volunteer in the investigation of modulating the CD226/TIGIT costimulatory axis for Regulatory T Cell therapies in the treatment of Type 1 Diabetes. He has since transitioned to a Student Research Assistant, starting in the Summer of 2021 with a role in Flow Activated Cell Sorting, flow cytometry staining and acquisition, assistance with immunological assays, and investigation of animal models. In Fall of 2021, Juan Martin began an Undergraduate Honors Thesis investigating the impact of T-cell Receptor Alpha Chain knock-outs on the phenotype and avidity of genetically modified regulatory T cells.
- Brown ME, Peters LD, Hanbali SR, Arnoletti JM, Sachs LK, Nguyen KQ, Carpenter EB, Seay HR, Fuhrman CA, Posgai AL, Shapiro MR, and Brusko TM (2022) Human CD4+CD25+CD226– Tregs Demonstrate Increased Purity, Lineage Stability, and Suppressive Capacity Versus CD4+CD25+CD127lo/- Tregs for Adoptive Cell Therapy. Front. Immunol. 13:873560. doi: 10.3389/fimmu.2022.873560
Abstracts & Presentations
- Arnoletti JM, Peters LD, Musca R, Sachs LK, Nguyen KQ, Meckler MP, Brusko TM. Effects of CRISPR/Cas9 mediated T Cell Receptor Knockout in Lentiviral Transduced CD4+ T cells to Improve Immunotherapies for Type 1 Diabetes. Poster Presented at: 2022 UF College of Medicine Research Day; 2022 Apr 4; Gainesville, FL.
- Brown ME, Arnoletti JM, Sachs LK, Nguyen KQ, Lee JJ, Brusko TM. CD226– Human Regulatory T Cells Exhibit Increased Stability Compared to Conventionally Sorted Populations. Poster presented at: 18th Congress of the Immunology of Diabetes Society (IDS); 2021 Nov 1-4; Virtual. (Outstanding Abstract Award Received)
- Brown ME, Hanbali SR, Gomez Rodriguez LM, Arnoletti JM, Carpenter EB, Brusko TM. Human CD4+CD25+CD226– Regulatory T Cells Demonstrate High Purity and Lineage Stability Following In Vitro Expansion for Adoptive Cell Therapy. Poster presented at: 81st Scientific Sessions of the American Diabetes Association (ADA); 2021 Jun 25-29; Virtual.
- Brown ME, Hanbali SR, Arnoletti JM, Brusko TM. Human CD226- T Regulatory Cells Exhibit High Purity and Linage Stability Following Ex Vivo Expansion for Adoptive Cell Therapy. Poster presented at: 2021 Annual University of Florida – College of Medicine Pediatrics Science Day; 2021 June 02; Virtual.
Lab: (352) 273-9300