Howie Seay, MS, LATg: Senior Biological Scientist, Laboratory Manager
Howie, Crasher, and Danielle
Howie joined the UF Diabetes Institute as part of the Brusko Lab in 2011 after giving back to society as a high school science teacher. His tasks in the lab include processing peripheral and cord blood samples, flow cytometric analysis of human immune cells and fluorescence-activated cell sorting, lab website and database maintenance, and the other various support duties of a research lab manager.
Howie’s current project involves isolating naïve regulatory T cells from cryogenically preserved umbilical cord blood units for expansion and potential re-infusion of autologous immune cells into children with Type 1 diabetes. We hope that this therapy can be used to repopulate the immune system with T cells that suppress autoimmunity and reverse the loss of insulin-producing ß-cells of the pancreas.
Howie is also working with the nPOD program on identifying the adaptive immune repertoire of T1D, as well as DNA methylation signatures in Pancreatic-draining lymph nodes in nPOD donors. These patterns can signify whether genes are turned on or off in a cell, and early evidence shows that cytotoxic T-lymphocytes in T1D patients are different at the epigenetic level when compared to healthy donor samples. The next step is determining why those differences happen and how those differences make the cells function in a unique way to aid in diabetes pathogenesis.
Lab Phone: (352) 273-9300
Mobile Phone: (352) 514-8839
Johns Hopkins University, Specialization Certificate, Data Science, 2016 (in progress)
Nova Southeastern University, M.S., Biomedical Informatics, 2015
University of Florida, B.S. Zoology, 2005
Global CpG methylation signature of pancreatic draining lymph node CD8+ T cells in type 1 diabetes. Howard Seay, Karl Kelsey, MD, Brock Christianson, PhD, Melissa Elliot, PhD, John Wiencke, PhD, Mark Atkinson, PhD, and Todd Brusko, PhD. 2013 nPOD Annual Meeting, Atlantic Beach, Florida, USA
Expansion of Cryopreserved Regulatory T-cells for Treatment of Type 1 Diabetes. Howard Seay, James Thompson, Christopher Fuhrman, Priya Saikumar Lakshmi, Phillip Lichlyter, Amy Patel, Amy Putnam, Jeffrey Bluestone, Mark Atkinson, Michael Haller, and Todd Brusko. 2013 Federation of Clinical Immunology Societies Annual Meeting, Boston, Massachusetts, USA.
Flow Cytometric Immunophenotyping of nPOD Donors.
Christopher Fuhrman, James Thompson, Howard Seay, and Todd Brusko
2012 nPOD Annual Meeting, Miami, Florida, USA
Yiming Yin, Seung-Chul Choi, Zhiwei Xu, Daniel J. Perry, Howard R. Seay, Byron P. Croker, Eric S. Sobel, Todd M. Brusko and Laurence Morel. “Normalization of CD4+ T cell metabolism reverses lupus” Science Translational Medicine. 2015 Feb 11;7(274):274ra18. doi: 10.1126/scitranslmed.aaa0835. PMID: 25673763
Sarkar, Debalina, Moanaro Biswas, Gongxian Liao, Howard R. Seay, George Q. Perrin, David M. Markusic, Brad E. Hoffman, Todd M. Brusko, Cox Terhorst, and Roland W. Herzog. “Ex vivo expanded autologous polyclonal regulatory T cells suppress inhibitor formation in hemophilia.” Molecular Therapy—Methods & Clinical Development 1 (2014).
O’Rourke, Jason R., Sara A. Georges, Howard R. Seay, Stephen J. Tapscott, Michael T. McManus, David J. Goldhamer, Maurice S. Swanson, and Brian D. Harfe. “Essential role for Dicer during skeletal muscle development.”Developmental biology 311, no. 2 (2007): 359-368.