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Howie Seay, MS, LATg: Biological Scientist II, Laboratory Manager

Danielle, Crasher, and Howie

Howie joined the UF Diabetes Institute as part of the Brusko Lab in 2011 after giving back to society as a high school science teacher. His tasks in the lab include processing peripheral and cord blood samples, flow cytometric analysis of human immune cells and fluorescence-activated cell sorting, lab website and database maintenance, and the other various support duties of a research lab manager.

Howie’s current project involves isolating naïve regulatory T cells from cryogenically preserved umbilical cord blood units for expansion and potential re-infusion of autologous immune cells into children with Type 1 diabetes. We hope that this therapy can be used to repopulate the immune system with T cells that suppress autoimmunity and reverse the loss of insulin-producing ß-cells of the pancreas.

Howie is also working with the nPOD program on identifying the adaptive immune repertoire of T1D, as well as DNA methylation signatures in Pancreatic-draining lymph nodes in nPOD donors. These methylation patterns can signify whether genes are turned on or off in a cell, and early evidence shows that cytotoxic T-lymphocytes in T1D patients are different at the epigenetic level when compared to healthy donor samples. The next step is determining why those differences happen and how those differences make the cells function in a unique way to aid in diabetes pathogenesis.

Contact Info

Mobile Phone: (352) 514-8839

Lab Phone: (352) 273-9300



  • Johns Hopkins University/Coursera, Specialization Certificate, Data Science, 2016 (in progress)

  • Nova Southeastern University, M.S., Biomedical Informatics, 2015

  • University of Florida, B.S. Zoology, 2005


Memberships & Activities

Oral Presentations

  • Global CpG methylation signature of pancreatic draining lymph node CD8+ T cells in type 1 diabetesHoward R. Seay, Karl Kelsey, MD, Brock Christianson, PhD, Melissa Elliot, PhD, John Wiencke, PhD, Mark Atkinson, PhD, and Todd Brusko, PhD. 2013 nPOD Annual Meeting, Atlantic Beach, Florida, USA

Poster Presentations