Select Journal Articles

Howard R. Seay, Amy L. Putnam, Judit Cserny, Amanda L. Posgai, Emma H. Rosenau, John R. Wingard, Kate F. Girard, Morey Kraus, Angela P. Lares, Heather L. Brown, Katherine S. Brown, Kristi T. Balavage, Leeana D. Peters, Ashley N. Bushdorf, Mark A. Atkinson, Jeffrey A. Bluestone, Michael J. Haller, and Todd M. BruskoExpansion of human Tregs from cryopreserved umbilical cord blood for GMP-compliant autologous adoptive cell transfer therapyNature Molecular Therapy — Methods & Clinical Development (In press).

Howard R. Seay, Erik Yusko, Stephanie J. Rothweiler, Lin Zhang, Amanda L. Posgai, Martha Campbell-Thompson, Marissa Vignali, Ryan O. Emerson, John S. Kaddis, Dave Ko, Maki Nakayama, Mia J. Smith, John C. Cambier, Alberto Pugliese, Mark A. Atkinson, Harlan S. Robins, Todd M. BruskoTissue distribution and clonal diversity of the T and B cell repertoire in type 1 diabetes.  JCI Insight. 2016 Dec 8; 1(20): e88242. PMCID: PMC5135280

Driver JP, Racine JJ, Ye C, Lamont DJ, Newby BN, McPhee-Leeth CG, Chapman HD, Brusko TM, Chen YG, Mathews CE, Serreze DV. Interferon gamma Limits Diabetogenic CD8 T-Cell Effector Responses in Type-1 Diabetes. Diabetes. 2016 Dec 5. pii: db160846. PMID: 27920091

Delitto D, Delitto AE, DiVita BB, Pham K, Han S, Hartlage ER, Newby BN, Gerber MH, Behrns KE, Moldawer LL, Thomas RM, George T, Brusko T, Mathews CE, Liu C, Trevino JG, Hughes SJ, Wallet S. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment. Cancer Res. 2016 Nov 18. pii: canres.1765.2016. PMID: 27864347

Haller MJ, Gitelman SE, Gottlieb PA, Michels AW, Perry DJ, Schultz AR, Hulme MA, Shuster JJ, Zou B, Wasserfall CH, Posgai AL, Mathews CE, Brusko TM, Atkinson MA, Schatz DA. Antithymocyte Globulin Plus G-CSF Combination Therapy Leads to Sustained Immunomodulatory and Metabolic Effects in a Subset of Responders With Established Type 1 Diabetes. Diabetes. 2016 Dec;65(12):3765-3775. PMID: 27669730

Choi SC, Hutchinson TE, Titov AA, Seay HR, Li S, Brusko TM, Croker BP, Salek-Ardakani S, Morel L. The Lupus Susceptibility Gene Pbx1 Regulates the Balance between Follicular Helper T Cell and Regulatory T Cell Differentiation.  J Immunol. 2016 Jul 15;197(2):458-69. doi: 10.4049/jimmunol.1502283. PMID: 27296664

Haller MJ, Atkinson MA, Wasserfall CH, Brusko TM, Mathews CE, Hulme M, Cintron M, Shuster J, McGrail K, Posgai A, Schatz D. Mobilization without immune depletion fails to restore immunological tolerance or preserve beta cell function in recent onset type 1 diabetes. Clin Exp Immunol. 2016 Mar;183(3):350-7. doi: 10.1111/cei.12731. PMID: 26462724

Song Xue, Amanda Posgai, Clive Wasserfall, Courtney Myhr, Martha Campbell-Thompson, Clayton E. Mathews, Todd Brusko, Alex Rabinovitch, Alexei Savinov, Manuela Battaglia, Desmond Schatz, Michael Haller, and Mark Atkinson. Combination therapy reverses hyperglycemia in NOD mice with established type 1 diabetes. Diabetes. 2015 Nov;64(11):3873-84. doi: 10.2337/db15-0164. PMID: 26185279

Christopher A. Fuhrman, Wen-I Yeh, Howard R. Seay, Priya Saikumar Lakshmi, Gaurav Chopra, Lin Zhang, Daniel J. Perry, Stephanie A. McClymont, Mahesh Yadav, Maria-Cecilia Lopez, Henry V. Baker, Ying Zhang, Yizheng Li, Maryann Whitley, David von Schack, Mark A. Atkinson, Jeffrey A. Bluestone, and Todd M. Brusko. Divergent Phenotypes of Human Regulatory T Cells Expressing the Receptors TIGIT and CD226. 20 May 2015, 10.4049/jimmunol.1402381

Yiming Yin, Seung-Chul Choi, Zhiwei Xu, Daniel J. Perry, Howard R. Seay, Byron P. Croker, Eric S. Sobel, Todd M. Brusko and Laurence Morel. “Normalization of CD4+ T cell metabolism reverses lupusScience Translational Medicine. 2015 Feb 11;7(274):274ra18. doi: 10.1126/scitranslmed.aaa0835. PMID: 25673763

Haller MJ, Gitelman SE, Gottlieb PA, Michels AW, Rosenthal SM, Shuster JJ, Zou B, Brusko TM, Hulme MA, Wasserfall CH, Mathews CE, Atkinson MA, Schatz DA. Anti-thymocyte globulin/G-CSF treatment preserves β cell function in patients with established type 1 diabetes. J Clin Invest. 2014 Dec 15. pii: 78492. doi: 10.1172/JCI78492. [Epub ahead of print] PMID: 25500887

Sarkar D, Biswas M, Liao G, Seay HR, Perrin GQ, Markusic DM, Hoffman BE, Brusko TM, Terhorst C, Herzog RW. Ex Vivo Expanded Autologous Polyclonal Regulatory T Cells Suppress Inhibitor Formation in Hemophilia. Mol Ther Methods Clin Dev. 2014 Jul 30;1. pii: 14030. PMID: 25364772

Lewis JS, Roche C, Zhang Y, Brusko TM, Wasserfall CH, Atkinson M, Clare-Salzler MJ, Keselowsky BG. Combinatorial delivery of immunosuppressive factors to dendritic cells using dual-sized microspheres. J Mater Chem B Mater Biol Med. 2014 May 7;2(17):2562-2574. PMID: 24778809

Altin Gjymishka, Roxana M Coman, Todd M Brusko, Sarah C Glover. Influence of host immunoregulatory genes, ER stress and gut microbiota on the shared pathogenesis of inflammatory bowel disease and Type 1 diabetes. Immunotherapy 12/2013; 5(12):1357-1366.

Christian Mueller, Jeffrey D. Chulay, Bruce C. Trapnell, Margaret Humphries, Brenna Carey, Robert A. Sandhaus, Noel G. McElvaney, Louis Messina, Qiushi Tang, Farshid N. Rouhani, Martha Campbell-Thompson, Ann Dongtao Fu, Anthony Yachnis, David R. Knop, Guo-jie Ye, Mark Brantly, Roberto Calcedo, Suryanarayan Somanathan, Lee P. Richman, Robert H. Vonderheide, Maigan A. Hulme, Todd M. Brusko, James M. Wilson and Terence R. Flotte. Human Treg responses allow sustained recombinant adeno-associated virus–mediated transgene expressionJ Clin Invest. doi:10.1172/JCI70314. Published November 15, 2013
Received for publication April 5, 2013, and accepted in revised form September 12, 2013.

Myhr CB, Hulme MA, Wasserfall CH, Hong PJ, Lakshmi PS, Schatz DA, Haller MJ, Brusko TM, Atkinson MA. The autoimmune disease-associated SNP rs917997 of IL18RAP controls IFNγ production by PBMC. J Autoimmun. 2013 Jul 24.

Thompson JA, Perry D, Brusko TM. Autologous Regulatory T Cells for the Treatment of Type 1 Diabetes. Curr Diab Rep. 2012 Jul 28. [Epub ahead of print] PubMed PMID: 22843491.

Hulme MA, Wasserfall CH, Atkinson MA, Brusko TM. Central role for interleukin-2 in type 1 diabetes. Diabetes. 2012 Jan;61(1):14-22.

Haller MJ, Wasserfall CH, Hulme MA, Cintron M, Brusko TM, McGrail KM, Sumrall TM, Wingard JR, Theriaque DW, Shuster JJ, Atkinson MA, Schatz DA. Autologous Umbilical Cord Blood Transfusion in Young Children With Type 1 Diabetes Fails to Preserve C-Peptide. Diabetes Care. 2011 Dec;34(12):2567-9. Epub 2011 Oct 19.

Sobel ES, Brusko TM, Butfiloski EJ, Hou W, Li S, Cuda CM, Abid AN, Reeves WH, Morel L. Defective response of CD4+ T cells to retinoic acid and TGFbeta in systemic lupus erythematosus. Arthritis Res Ther. 2011 Jun 27;13(3):R106. [Epub ahead of print] PubMed PMID: 21708033.

McClymont SA, Putnam AL, Lee MR, Esensten JH, Liu W, Hulme MA, Hoffmüller U, Baron U, Olek S, Bluestone JA, Brusko TM. Plasticity of human regulatory T cells in healthy subjects and patients with type 1 diabetes. J Immunol. 2011 Apr 1;186(7):3918-26. Epub 2011 Mar 2.

Golovina TN, Mikheeva T, Brusko TM, Blazar BR, Bluestone JA, Riley JL. Retinoic acid and rapamycin differentially affect and synergistically promote the ex vivo expansion of natural human T regulatory cells. PLoSOne. 2011 Jan 6;6(1):e15868.

Brusko TM, Koya RC, Zhu S, Lee MR, Putnam AL, McClymont SA, Nishimura MI, Han S, Chang LJ, Atkinson MA, Ribas A, Bluestone JA. Human antigen-specific regulatory T cells generated by T cell receptor gene transfer. PLoS One. 2010 Jul 22;5(7):e11726.

Brusko, TM, Clive Wasserfall, C.H., Hulme M., Cabrera R., Schatz D., and M.A. Atkinson. Influence of Membrane CD25 Stability on T Lymphocyte Activity: Implications for Immunoregulation. PLoS ONE. 2009 Nov 24;4(11):e7980.

*A.L. Putnam, *Brusko TM, M.R. Lee, W. Liu, G.L. Szot, T. Ghosh, M.A. Atkinson, and J.A. Bluestone. Expansion of Human Regulatory T Cells from Patients with Type 1 Diabetes. Diabetes, 2009 Mar;58(3):652-62. Epub 2008 Dec 15. *Co-first author

Brusko TM, Bluestone JA. Regulatory T cells directed to the site of the action.Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20553-4. Epub 2009 Dec 1.

Haller MJ, Wasserfall CH, McGrail KM, Cintron M, Brusko TM, Wingard JR, Kelly SS, Shuster JJ, Atkinson MA, Schatz DA. Autologous umbilical cord blood transfusion in very young children with type 1 diabetes. Diabetes Care. 2009 Nov;32(11):2041-6.

Brusko TM. Mesenchymal stem cells: a potential border patrol for transplanted islets? Diabetes. 2009 Aug;58(8):1728-9.

Xue S, Wasserfall CH, Parker M, Brusko TM, McGrail S, McGrail K, Moore M, Campbell-Thompson M, Schatz DA, Atkinson MA, Haller MJ. Exendin-4 therapy in NOD mice with new-onset diabetes increases regulatory T cell frequency. Ann N Y Acad Sci. 2008 Dec;1150:152-6.

Stalvey MS, Brusko TM, Mueller C, Wasserfall CH, Schatz DA, Atkinson MA, Flotte TR. CFTR mutations impart elevated immune reactivity in a murine model of cystic fibrosis related diabetes. Cytokine. 2008 Oct;44(1):154-9. Epub 2008 Sep 7.

Brusko TM, Amy L. Putnam, and Jeffrey A. Bluestone. Human regulatory T cells; Role in autoimmune disease and therapeutic opportunities. Immunol. Rev. 2008 Jun;223:371-90.

Brusko, T.M. and Jeffrey Bluestone. The clinical application of Tregs for the treatment of type 1 diabetes and transplantationEur J Immunol. 2008 Apr 8;38(4):931-934.

Brusko, T.M.,Clive Wasserfall,Kieran McGrail,Richard Schatz, Hilla Lee Viener,Desmond Schatz,Michael Haller,JenniferRockell, Peter Gottlieb, Michael Clare-Salzler, and Mark AtkinsonNo Alterations in the Frequency of FOXP3+ Regulatory T Cells in Type 1 DiabetesDiabetes. 2007 Mar;56(3):604-12.

Lowe CE, Cooper JD, Brusko T.M., Walker NM, Smyth DJ, Bailey R, Bourget K, Plagnol V, Field S, Atkinson M, Clayton DG, Wicker LS, Todd JA. Large-scale genetic fine mapping and genotype-phenotype associations implicate polymorphism in the IL2RA region in type 1 diabetesNat Genet. 2007 Sep; 39(9):1074-1082. Epub 2007 Aug 5.

Brusko T.M.,Maigan A. Hulme, Courtney B. Myhr, Michael J. Haller, and Mark A. Atkinson. Assessing the In Vitro Suppressive Capacity of Regulatory T CellsImmunol Invest. 2007;36(5):607-28.

Brusko TM, C. H. Wasserfall, M. J. Clare-Salzler, D. A. Schatz, and M. A. Atkinson. 2005. Functional defects and the influence of age on the frequency of CD4+CD25+ T-cells in type 1 diabetes. Diabetes 54:1407-1414.

Brusko TM, C. H. Wasserfall, A. Agarwal, M. H. Kapturczak, and M. A. Atkinson. 2005. An integral role for heme oxygenase-1 and carbon monoxide in maintaining peripheral tolerance by CD4+CD25+ regulatory T cellsJ.Immunol. 174:5181-5186.

Goudy, K. S., B. R. Burkhardt, C. Wasserfall, S. Song, M. L. Campbell-Thompson, Brusko T.M., M. A. Powers, M. J. Clare-Salzler, E. S. Sobel, T. M. Ellis, T. R. Flotte, and M. A. Atkinson. 2003. Systemic overexpression of IL-10 induces CD4+CD25+ cell populations in vivo and ameliorates type 1 diabetes in nonobese diabetic mice in a dose-dependent fashionJ.Immunol. 171:2270-2278.

Haller, M. J., M. Samyn, W. W. Nichols, Brusko T.M., C. Wasserfall, R. F. Schwartz, M. Atkinson, J. J. Shuster, G. L. Pierce, and J. H. Silverstein. Radial artery tonometry demonstrates arterial stiffness in children with type 1 diabetesDiabetes Care 27:2911-2917.

Kapturczak, M. H., C. Wasserfall, Brusko T.M., M. Campbell-Thompson, T. M. Ellis, M. A. Atkinson, and A. Agarwal. Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouseAm.J.Pathol. 165:1045-1053, 2004.

Morales, A., C. Wasserfall, Brusko T.M., C. Carter, D. Schatz, J. Silverstein, T. Ellis, and M. Atkinson. Adiponectin and leptin concentrations may aid in discriminating disease forms in children and adolescents with type 1 and type 2 diabetesDiabetes Care 27:2010-2014, 2004.